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KMID : 0903520090520010017
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 1 p.17 ~ p.21
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Cellulolytic Enzymes Production from Submerged Fermentation of Different Substrates by Newly Isolated Bacillus spp. FME
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Kumar G.Satheesh
Chandra M.Subhosh
Sumanth M.
Vishnupriya A.
Reddy B.Rajasekhar
Choi Yong-Lark
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Abstract
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Newly isolated strains Bacillus sp. FME 1 and FME 2 were evaluated for the cellulolytic enzymes production during submerged fermentation (SmF) of different substrates including rice husk, Whatman filter paper and cellulose powder CF 11. Extracellular enzyme assays for CMCase, FPase and β-glucosidase were examined up to 8 days of submerged fermentation. Among the three substrates, rice husk was the most suitable substrate for higher production of cellulolytic enzymes. Maximum titers of 100, 45, and 3.5 U/mL in respect of CMCase, FPase and β-glucosidase in Bacillus sp. FME 2 were recovered as against 45, 12, and 0.39 U/mL in Bacillus sp. FME 1 respectively, at their respective peak time intervals. Bacillus sp. FME 2 was found to produce higher cellulolytic enzyme activities than Bacillus sp. FME 1.
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KEYWORD
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Bacillus sp., cellulolytic enzymes, cellulose powder CF 11, rice husk, submerged fermentation, Whatman filter paper
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